الصفحة الرئيسية

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السيرة الذاتية
نسبة اكتمال الملف الشخصي
الجنسية
التخصص العام
التخصص الدقيق
المسمى الوظيفي
الدرجة العلمية (المرتبة)

نبذه مختصرة

My research interests are the bioadhesion of endothelial cells onto functionalized biomaterials In my PhD thesis, I have investigated the parameters that determine cell interactions - materials depend on the properties of the biomaterial surface and the intrinsic properties of cells The objective is to characterize the physicochemical properties, morphological and electrical surface before and after functionalization and to correlate these properties with the adhesion energy of cells on these materials I have particularly used the electrochemical impedance spectroscopy (EIS) technique which is an effective tool for monitoring endothelial cell behaviour on a fibronectin/ polystyrene/thiol/gold electrode I have also studied the electrical properties of endothelial cell monolayer immobilized on fibronectin/polystyrene/thiol/ gold electrode using the impedance spectroscopy When endothelial cells attach and spread on the surface of a biofunctionnalized gold electrode, they behave essentially like insulating particles that hinder unrestricted current flow from the electrode into the bulk electrolyte and thereby increase the overall electrode impedance Recently, I am mainly interested on the study of Endothelial Cells Adhesion onto functionnalized substrates Using Surface Plasmon Resonance The focus of this work will be on the endothelial cell monolayer immobilized adhesion onto functionnalised substrates such as fibronectin/polystyrene/thiol/ gold electrodes, by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM) The obtained results have been confirmed by impedance measurements and wettability studies

المؤهلات العلمية

* December 2006 : PhD in Biotechnology from the University of Monastir in Tunisia and also from the « Ecole Centrale de Lyon » in France Research theme: "Characterization and quantification of endothelial cell adhesion to biomaterials functionalized * December 2002 : Master of Biology (Postgraduate Degree, granted 6 years after the baccalaureat), in the Faculty of Sciences of Monastir, Monastir University, Tunisia Research theme : « Effect of flow on the deformability of endothelial cells and red blood cells» * Juin 1999 : Bachelor of Sciences - Biology (Postgraduate Degree, granted 4 years after the baccalaureat), in the Faculty of Sciences of Tunis, Tunis El-Manar University, Tunisia

الاهتمامات البحثية

My research interests are the bioadhesion of endothelial cells onto functionalized biomaterials In my PhD thesis, I have investigated the parameters that determine cell interactions - materials depend on the properties of the biomaterial surface and the intrinsic properties of cells The objective is to characterize the physicochemical properties, morphological and electrical surface before and after functionalization and to correlate these properties with the adhesion energy of cells on these materials I have particularly used the electrochemical impedance spectroscopy (EIS) technique which is an effective tool for monitoring endothelial cell behaviour on a fibronectin/ polystyrene/thiol/gold electrode I have also studied the electrical properties of endothelial cell monolayer immobilized on fibronectin/polystyrene/thiol/ gold electrode using the impedance spectroscopy When endothelial cells attach and spread on the surface of a biofunctionnalized gold electrode, they behave essentially like insulating particles that hinder unrestricted current flow from the electrode into the bulk electrolyte and thereby increase the overall electrode impedance Recently, I am mainly interested on the study of Endothelial Cells Adhesion onto functionnalized substrates Using Surface Plasmon Resonance The focus of this work will be on the endothelial cell monolayer immobilized adhesion onto functionnalised substrates such as fibronectin/polystyrene/thiol/ gold electrodes, by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM) The obtained results have been confirmed by impedance measurements and wettability studies

الخبرات والمناصب الإدارية

- President of the Quality Committee in the Biology Department - Member in the Quality Committee in the Faculty of Sciences - Member in the Quality Committee in the Deanship of Quality and Development Membership in the Saudi Biological Society (SBS)  Member of the Standing Committee of the planning and the final wording at the Deanship of Quality and Development, University of Tabuk  Consultant in the Academic Accreditation (Female Section) at the Deanship of Quality and Development, University of Tabuk  Member of the Committee of Academic Accreditation at the Department of Biology (Female Section)  Member of the Graduate Studies and Research Committee  Member of the Study Plans Committee

الجدول الدراسي
اليوم المادة الوقت
من إلى
الأحد BIOD0531 شعبة 71 16:00 18:00
الإثنين ACES1104 شعبة 95 08:00 09:30
الإثنين ACES1104 شعبة 183 11:00 12:30
الثلاثاء BIOD0531 شعبة71 16:00 18:00
الأربعاء ACES1104 شعبة 95 08:00 09:30
الأربعاء ACES1104 شعبة 183 11:00 12:30
الأبحاث والمؤلفات
  • 1/ A Bouafsoun, A Othmane, A Kerkeni, N Jaffrézic , L Ponsonnet, « Evaluation of endothelial cell adherence onto collagen and fibronectin: A comparison between jet impingement and flow chamber techniques, Materials Science and Engineering: C 26 (2006),260-266 2/ Amira Bouafsoun, Saloua Helali, Saida Mebarek, Caroline Zeiller, Annie-France Prigent, Ali Othmane, Abdelhamid Kerkeni, Nicole Jaffrézic Renault and Laurence Ponsonnet, “Electrical probing of endothelial cell behaviour on a fibronectin/polystyrene/thiol/gold electrode by faradaic electrochemical impedance spectroscopy (EIS), Bioelectrochemistry 71 (2006), 99-105 3/ Amira Bouafsoun, Ali Othmane, Abdelhamid Kerkeni, Nicole Jaffrézic Renault and Laurence Ponsonnet, “Comparative wettability study of Polystyrene functionnalized with different proteins, Materials Science and Engineering: C 27 (2007), 709-715 4/ Amira Bouafsoun, Saloua Helali, Ali Othmane, Abdelhamid Kerkeni, Annie-France Prigent, Nicole Jaffrézic-Renault, François Bessueille, Didier Léonard, Laurence Ponsonnet, “Evaluation of endothelial cell adhesion onto different protein gold electrodes by Faradaic electrochemical impedance spectroscopy Macromolecular Biosciences 7 (2007), 599-610 5/ Amira Bouafsoun, Ali Othmane, Nicole Jaffrézic Renault, Abdelhamid Kerkeni, Olivier Thoumine, Annie-France Prigent and Laurence Ponsonnet, “Impedance endothelial cell biosensor for lipopolysaccharide detection, Materials Science and Engineering C, 287 (2008), 653-661 6/ Amira Bouafsoun, Nicole Jaffrézic Renault, Laurence Mora, “Spectroscopy Resonance Plasmon Efficient Tool for Cell Adsorption, Journal Of NanoReseach, 59 (2019), 35-45 INTERNATIONAL CONFERENCES 1/ A Bouafsoun, A Othmane, A Kerkeni, H Ben Ouada, J Dufaux «Technique physique de mesure de la déformation des particules soumises à un flux – Application : cellules endothéliales et globules rouges » 5th Edward A Bouchet International Conference on Physics and high Technology, 11 - 15 August 2003 in Hammamet, Tunisia (Oral) 2/ A Bouafsoun, S Helali, A Othmane, A Kerkeni, L Ponsonnet, N Jaffrezic, F Bessueille, D Léonard « Evaluation of endothelial cell adherence onto proteins coated polystyrene by electrochemical impedance spectroscopy», Invited speaker to International Meeting, THERMEC 2006, Biomaterials Symposium, 4-8 July 2006, Vancouver, CANADA (Oral) 3/ A Bouafsoun, A Othmane, A Kerkeni, N Jaffrezic-Renault, L Ponsonnet ,EIS for endothelial cell behaviour: effect of fibronectin treatment and sensor response to LPS,International Meeting, MADICA 2006, 30 October-01 November 2006, Mahdia, Tunisiea (Oral) 4/ Amira Bouafsoun, Ali Othmane, Abdelhamid Kerkeni, Nicole Jaffrézic, Laurence Ponsonnet, «Endothelial cell adhesion to functionalized biomaterials » Invited Speaker to International Meeting “Impact of metallurgy on economy, development and environment (Cime_07) 23 – 25 Avril 2007 à Annaba, Algeria (Oral) 5/ A Bouafsoun, A Othmane, A Kerkeni, H Ben Ouada, J Dufaux « Effet de l’écoulement sur la déformabilité des cellules endothéliales et des globules rouges », International Meeting, 5th Edward A Bouchet International Conference on Physics and high Technology, du 11 au 15 August 2003 à Hammamet, Tunisia (Poster) 6/ A Bouafsoun , A Othmane, A Kerkeni, N Jaffrezic – Renault, L Ponsonnet " Etude comparative de differentes méthodes de détermination de la mouillabilité : application à des biomatériaux fonctionnalisés ", International Meeting, 7ème Journées Francophones des Jeunes Physico-Chimistes (7JFJPC), 19-21 March 2004, Monastir, Tunisia (Poster) 7/ A Bouafsoun, A Othmane, A Kerkeni, N Jaffrezic – Renault, L Ponsonnet : « Evaluation de l’adhésion de la cellule endothéliale à des surfaces fonctionnalisées International Meeting , MADICA 2004, 29 November-1 December 2004, Tunis, Tunisia (Poster) 8/ A Bouafsoun, A Othmane, H Ben Ouada, A Kerkeni, N Jaffrezic, L Ponsonnet, « Evaluation de l’adhésion des cellules endothéliales au polystyrène fonctionnalisé par différentes protéines », International Meeting, JADH 2005, September 2005, Mulhouse, France (Poster) 9/ A Bouafsoun, A Othmane, A Kerkeni, L Ponsonnet, N Jaffrezic, « Evaluation et caractérisations de l’adhésion des cellules endothéliales aux polymères», International Meeting, ARATEM 2006, Saint-Etienne 15 February 2006, France (Poster) 10/ A Bouafsoun, S Helali, Saida Mebarek, Caroline Zeiller, Annie-France Prigent, Ali Othmane, Abdelhamid Kerkeni, Laurence Ponsonnet et Nicole Jaffrézic Renault, « The electrochemical impedance spectroscopy (EIS) : A sensitive indicator of the endothelial cell behaviour on fibronectin/polystyrene/thiol/gold electrodes », International Meeting , Biosensors 2006, 12-12 May 2006, Toronto, CANADA (Poster) 11/ A Bouafsoun, A Othmane, A Kerkeni, N Jaffrezic, L Ponsonnet, « Evaluation of endothelial cell : A comparison between jet impingement and flow chamber techniques», International Meeting « European Society for Biomaterials Conference 2006» ESB 2006, 27 September-01 October 2006, NANTES, France (Poster) 12/ A Bouafsoun, S Helali, A Othmane, A Kerkeni, N Jaffrézic-Renault, F Bessueille, D Leonard, L Ponsonnet, « Evaluation of endothelial cell adhesion onto different protein/gold electrodes by Faradaic electrochemical impedance spectroscopy », International Meeting « MADICA 2006» 30 October-01 November 2006, Mahdia, Tunisia (Poster) 13/ S Helali, Hben Fredj, A Bouafsoun, A Abdelghani, « Polypyrrole and Polyaniline: comparative study for biosensor application », International Meeting, MADICA 2008, Maroc (Oral) 14/ A Bouafsoun, A Abdelghani, L Mora, “ Studying of Endothelial Cells Adhesion onto functionnalized substrates Using Surface Plasmon Resonance », International Meeting, « Nanoscale Science and Technology », 17-19 March 2012, Hammamet, Tunisia (oral)
جوائز التميز
  • تحصلت على جائزة التمييز في السنة الجامعية 1440/1441
المشاريع البحثية
اسم المشروع وصف المشروع
Studying of Endothelial Cells Adhesion onto functionalized substrates for biomedical and biological applications S-0137-1438 Submitted
معلومات التواصل
البريد الإلكتروني :

الخصوصية وملفات تعريف الارتباط
هذا الموقع يستخدم ملفات تعريف الارتباط الخاصة للتأكد من سهولة الاستخدام وضمان تحسين تجربتك أثناء التصفح. من خلال الاستمرار في تصفح هذا الموقع، فإنك تقر بقبول استخدامنا لملفات تعريف الارتباط. الشروط والأحكام.